Lihlahisoa

Lintlha tsa Sehlahisoa:

Teko ea Innovita® 2019-nCoV IgM/IgG e etselitsoe tlhahlobo le tlhahlobo ea mafu a bakoang ke "coronavirus" e ncha (2019-nCoV).ORF1ab le N gene tsa 2019-nCoV li bonoa ka mokhoa o nepahetseng ho tsoa ho li-swabs tsa 'metso le lisampole tsa alveolar lavage fluid tse bokelletsoeng maemong a belaelloang a pneumonia, bakuli ba nang le linyeoe tse belaelloang, ba bang ba hlokang ho hlahlojoa.
Liphetho tsa tlhahlobo ea khithi ena ke tsa litšupiso tsa bongaka feela.Ho khothalletsoa ho etsa tlhahlobo e felletseng ea boemo bo ipapisitseng le lipontšo tsa kliniki tsa mokuli le liteko tse ling tsa laboratori.

Molao-motheo:

Sesebelisoa sena se sebelisa mohato o le mong oa reverse transcript polymerase chain reaction (RT-PCR) theknoloji ea ho lemoha ho shebana le mofuta oa "coronavirus" (2019-nCoV) ORF1ab gene, N gene, le tatellano ea liphatsa tsa lefutso tsa motho.Li-primers tse khethehileng le li-probe tsa taqman li entsoe libakeng tse sirelelitsoeng.

Moetso:

Tlhokomeliso: 1. Mefuta e fapaneng ea li-reagents ha ea lokela ho kopanngoa.
2. Litaolo tse ntle le tse ntle ha lia lokela ho ntšoa

Mokhoa oa Teko:

1. Ho ntšoa ha asiti ea nucleic:
Lisebelisoa tsa ho hula tsa RNA tsa khoebo lia fumaneha, ho ntšoa ha lifaha tsa makenete le ho ntšoa ha melamo ea spin ho khothaletsoa bakeng sa khithi ena.
2. Lokisetsa Rection Mix:

● Ntsha 2019-nCoV reaction Mix A/B mme o boloke mocheso wa kamore ho fihlela o sa hatsele;
● Nka likarolo tse lumellanang (Reaction Mix A 16.5μL/T, Reaction Mix B 3.5μL/T) 'me u kopanye, 'me joale aliquot karabelo e' ngoe le e 'ngoe ea PCR ka 20μL / tube;
● Eketsa 5μL ea template ea RNA kapa taolo e mpe kapa taolo e ntle, ebe u koahela cap cap;
● Beha tšupu ea karabelo sesebelisoa sa PCR sa fluorescence, 'me u sete taolo e mpe / e nepahetseng le litekanyetso tsa sampole bakeng sa karabelo ea RT-PCR ho latela litaelo tsa ts'ebetso ea sesebelisoa.
● Rekota Odara ea ho beha mohlala

3.RT-PCR protocol:

Litlhophiso tse khothalelitsoeng: