Teko ea 2019-nCoV Neutralizing Antibody (QDIC)
Lintlha tsa Sehlahisoa:
Teko ea Innovita® 2019-nCoV IgM/IgG e etselitsoe ho lemoha ka bongata ha anti-antibody e sa thekeseleng ho coronavirus e ncha (2019-nCoV) ho serum ea motho, plasma kapa mali a felletseng (mali a monoana kapa mali a venous kaofela).
2019-nCoV e kenyelletsa liprotheine tse 'ne tsa mantlha tsa sebopeho: S protheine, E, protheine ea M le protheine ea N.Sebaka sa RBD sa protheine ea S se ka hokahana le ACE2 ea sele ea motho.Boithuto bo bonts'itse hore mehlala ea batho ba folileng ts'oaetso ea "coronavirus" e ncha e ntle bakeng sa ho fokotsa li-antibody.Ho sibolloa ha li-anti-antibody tse fokolang ho ka sebelisoa ho lekola boemo ba tšoaetso ea kokoana-hloko le tlhahlobo ea phello ka mor'a ho entoa.
Molao-motheo:
Kit ke tlhahlobo ea quantum dot immunofluorescence chromatography assay ho bona li-antibodies tsa 2019-nCoV RBD tse ikhethileng tsa IgG ho serum ea motho, plasma kapa mali a felletseng (mali a monoana le mali a venous kaofela).Ka mor'a hore mohlala o sebelisoe ho mohlala hantle, haeba motsoako oa li-antibodies tse fokolang li phahame ho feta tekanyo e tlaase ka ho fetisisa ea ho lemoha, li-antibodies tsa RBD tse khethehileng tsa IgG li tla itšoara ka karolo kapa antigen eohle ea RBD e ngotsoeng ka quantum dot microspheres ho theha motsoako oa 'mele oa ho itšireletsa mafung.Ebe motsoako oa 'mele oa ho itšireletsa mafung o tla falla hammoho le lera la nitrocellulose.Ha ba fihla sebakeng sa teko (T line), motsoako o tla itšoara ka mouse e khahlanong le motho IgG (γ chain) e koahetsoeng ka lera la nitrocellulose ebe e etsa mohala oa fluorescent.Bala boleng ba lets'oao la fluorescence ka sesebelisoa sa fluorescence immunoassay.Boleng ba lets'oao bo ipapisitse le litaba tsa li-antibodies tse fokolang setšoantšong.
Ho sa tsotellehe hore na mohlala o na le li-antibodies tse khethehileng tsa RBD kapa che, mohala oa taolo o lokela ho lula o hlaha fensetereng ea sephetho haeba tlhahlobo e etsoa hantle 'me reagent e sebetsa kamoo e reriloeng kateng.Ha kokoana ea IgY antibody e ngotsoeng ka quantum dot microspheres e fallela moleng oa taolo (C line), e tla tšoaroa ke poli ea anti-chicken IgY antibody e pota-potiloeng moleng oa C, 'me mohala oa fluorescent o thehoa.Mohala oa taolo (C line) o sebelisoa e le taolo ea tsamaiso.
Moetso:
| Sebopeho | Chelete | Tlhaloso |
| IFU | 1 | / |
| K'hasete ea teko | 20 | Mokotla o mong le o mong o tiisitsoeng oa foil o nang le sesebelisoa se le seng sa liteko le desiccant e le 'ngoe |
| Setšoantšo se hlapollotsoeng | 3mL * 1 pial | 20mM PBS, Sodium Casein, ProClin 300 |
| Micropipette | 20 | Micropipette e nang le mohala oa 20μL |
| Lancet | 20 | / |
| Letlapa la joala | 20 | / |
Mokhoa oa Teko:
● Pokello ea Mali ea Monoana
● Bala sephetho ka mochine oa fluorescence analyzer
